Transcript of Pasteurella multocida microbiology

asalam alaikum everybody I hope you all are doing well Today we'll be talking about an organism that is responsible for causing wound infections associated with cat and dog bites It's none other than pasteurella moltoida But before getting into the video I'd like to tell you guys that these videos are meant for educational purposes Things and treatments may change with time If I get wrong or miss anything your input is always welcomed in the comment section So have a cup of tea and let's get started Pasteurella multraidera It's a gram negative rod or a cocoa basilus Cocoa basilus is a shape somewhere between the spherical caucus and the rod-shaped basilus So you will see sometimes it like a rod or both together It's an encapsulated rod It is short bacterium and is responsible for causing wound infections associated with cats and dogs bites This is how pasteurella multicider looks like under the microscope But before talking about pasteurella multicider in much detail we should know about bacterial classification Bacteria are further classified into spyit They're also classified into acidfast based on acid fast staining And there's an exception that is microplasma bacterium Bacteria are also classified based on gram staining into gram positive We're done with all of them If you're interested be sure to check out the channel and gram negative and gram negative bacteria are further subdivided into cooxy which includes niceria neria ganora and niceria manager to this and also into rods which are further subdivided into aerobic like sudamonus anorobic like bacteroides and fusobacterium and facultative which is further subdivided into curved that includes campilobacttor helicoacttor and vibrio and into straight which is further Subdivide you into teran related that includes E.coli interobacttor seratia clapsella salmonella chagela and proteus into respiratory that includes hemophilus bordella and legionella and lastly into zenotic which includes brusella francicella pasteurella the topic of today's video and your senior We are done with almost all of the gram negative bacteria So if you're interested be sure to check out the channel lecture outline We are done with the introduction of pastrial multraida We are done with the bacterial classification Now we'll be looking at morphology habitat in transmission pathogenesis clinical findings lab diagnosis treatment prevention and at the end as usual we'll review the lecture A huge thanks to True Learn for sponsoring today's video True Learn is an incredible online exam preparation resource This platform is built for medical students nurses and professionals It helps you prepare for USML Comlex ANCLEX and other exams Getting started is super easy Just click the link below in the description This is the beautiful website of Trulon Scroll it down You can also check their plans and choose the one that you like Hit buy now and use my special coupon code Medzor at the checkout to get an exclusive discount Login This is the True Learns dashboard For creating the test click the create test right there Give it a name Choose a time limit Select if you want a tutor and also the difficulty level Once you're done just hit start test and your test is ready to go Read the question select your answer and see how you did Trulan explains the answer very well Just like that So everybody if you're interested in signing up on Trulan click the link below and don't forget to use my special discount code meds at the checkout Sign up and enjoy Morphology shape As we've discussed earlier in the introduction section that pasteurella moltoida is rodshaped cocoa basilus It is pink colored The reason is it's scrum negative and it exhibits a bipolar staining which means it stains at both poles means both ends but it does not stain from the central area structure It's an encapsulated bacterium It is not responsible for forming spose and is nonmotile This is how pasturella multicider looks like under the microscope We do not see any motility apparatus like a fledgula That's why the spectrum is non-mile It is pink colored The reason is it's scrum negative Habitate hosts Humans are its hosts and it's also found in some animals like it's the part of normal flora of mouth of domestic animals like cats and dogs I've put a cute picture of cat and dog here so you can memorize that pastela is found in these animals Transmission It is transmitted by biting bites of animals like cats and dogs Pathogenesis I've put a handshake of human and cat here which can help you memorize that this infection is transmitted by cats to humans This is the cat You can see its mouth has got sharp and pointed teeth and pasturella multraid is found in the normal mouth flora of the cats So when cow will bite the humans it sharp and pointed teeth will implant the pasturella multraida under the perryioium Okay Now let me tell you where is perryioium On top of everything humans have skin Skin has three layers Top one is epidermis then dermis and then hypodermis The last layer of the skin is also termed as the subcutaneous tissue After these three layers of the skin comes the perryostium which is a membrane covering the outer surface of the bone So look how sharp and pointed the cat's teeth are that they implant the pasteurella under the perryioium like under that membrane And you know what sutures are the predisposing factors to the infection Most white infections are polyicrobial Now you might be asking me a question What is polyicrobial okay it has got two words in it First one is poly which means many and the second one is microbial which means microbes So these bite infections have a variety of facultative anorobpecially streptococcus species and anorobic organisms in addition to pastriala maltocida The capsule of this bacterium is a verness factor It also has an endotoxin that's present in its cell wall which also a vance factor Clinical findings A rapidly spreading cellulitis at the site of an animal bite is indicative of pastural mulic infection Incubation period is brief usually less than 24 hours Osteomiitis can complicate cat bite because cats sharp and pointed teeth can implant the organism under the perryostium which is the membrane that covers the outer surface of the bone Symptoms include pain swelling redness tenderness warmth at the sight of the infection and the patient may feel fever Lab diagnosis We'll need a sample from the wound site like the site where the cat or dog has bitten Then we'll go for microscopy and on gram staining This bacterium appears to be gram negative It's rod or cocoa basilus in shape It is pink or red colored cuz it's gram negative and exhibits bipolar staining which means it stains at both poles but it doesn't stain from the central area This is how pasturella multicider looks like under the microscope It's pink colored Why because it's gum negative Treatment Panicellin G is a treatment of choice There's no significant antibiotic resistance People who have been bitten by a cat should be given empellin to prevent prior multicider infection Prevention Avoid contact with animals such as cats to avoid their bites Animal bites especially cat bites should not be suited because they can complicate the situation All right everybody let's have a quick recap The organism we discussed today is pastela malttoida It responsible for causing pastrolosis It is transmitted by animal bites Hosts include humans cats and dogs Diagnosis is based on gram staining and microscopy And for treatment penicellin G is the drug of choice And that's it for today's video I hope you enjoyed it You've learned pastrial multicider in detail If you've got any suggestions feel free to leave them below in the comments I'd be happy to read them And I'll catch you soon in the next video And if you want to connect with me on my social media I've got my Instagram and Twitter both with the handle med And I'll catch you soon in the next video Till then asalamu alayikum